Protein extraction and sample preparation

RIPA lysis buffer is a traditional tissue and cell rapid lysis buffer, mainly used to extract soluble proteins from animal tissues and mammalian cells, and can be used to lyse adherent cells and suspended cells. Different levels of RIPA lysis buffers, weak, medium, and strong, can be used for protein extraction according to the requirements of protein extraction. Proteins extracted with RIPA lysis buffer can be used for protein quantification, Western blot, IP, etc., and are particularly suitable for Co-IP detection.

Plant tissue protein extraction reagent can be used to extract total protein from plant samples. The plant tissue protein extracted using this reagent can be used for downstream operations such as protein activity analysis, immunoassay, protein purification, etc., and protein quantification can be performed using BCA, Bradford, and Lowry methods.

The Animal Tissue Protein Extraction Kit can be used to extract total protein from animal tissue samples. The tissue protein extracted using this extract can be used for downstream operations such as protein activity analysis, immunoassay, protein purification, and protein quantification using BCA, Bradford, and Lowry methods. This kit contains a protease inhibitor cocktail and PMSF, which can effectively avoid protein degradation during the extraction process.

This kit adds a mixture of protease inhibitors to the bacterial protein extraction buffer. The extracted protein retains its biological activity and can be used for downstream experimental operations such as IP, Western blot, and protein purification.