lfe sciences
Plant one millet in spring and harvest ten thousand millets in autumn. Pangu quantitative analysis helps germplasm resource genomics research
Plant one millet in spring and harvest ten thousand millets in autumn. Pangu quantitative analysis helps germplasm resource genomics research
2024-04-26
Understanding Germplasm Resources
Geroplasm resources, also known as genetic resources or gene resources, refer to genetic materials that have actual or potential value for human use. Plant resources such as seeds, seedlings and tissues for crop genetic improvement and variety improvement, and animal resources with excellent varieties all fall within the scope of germplasm resources.
my country has a vast territory and extremely rich germplasm resources, ranking first in the world. Agricultural germplasm resources such as crops, livestock and poultry, aquatic products, and agricultural microorganisms are the basis for the selection and breeding of new varieties. They are the carriers of genetic information from the previous generation to the next generation. Germplasm resources are one of the important resources indispensable for human survival.
Therefore, my country has been committed to taking multi-dimensional measures to protect and develop germplasm resources and realize agricultural modernization.
On January 1, 2024, the Ministry of Agriculture’s No. 1 document "Opinions on Learning and Using the Experience of the "Thousand Villages Demonstration and Ten Thousand Villages Renovation" Project to Effectively Promote the Comprehensive Revitalization of Rural Areas"
The document proposes the "Seed Industry Revitalization Action", which includes the following:
1. Strengthen the protection and utilization of germplasm resources, screen and explore excellent germplasm and gene resources such as soybeans and corn, and promote breeding innovation.
2. Accelerate the industrialization of biological breeding, and accelerate the selection and promotion of independent excellent varieties urgently needed for production, such as high-oil and high-yield soybeans, short-growing rapeseed, and salt-alkali-tolerant crops.
my country has a vast territory and extremely rich germplasm resources, ranking first in the world. Agricultural germplasm resources such as crops, livestock and poultry, aquatic products, and agricultural microorganisms are the basis for the selection and breeding of new varieties. They are the carriers of genetic information from the previous generation to the next generation. Germplasm resources are one of the important resources that are indispensable for human survival.
Germplasm resources and genomics
The protection of biological germplasm resources is usually inseparable from modern scientific and technological infrastructure, including seed banks, plant in vitro banks, DNA banks, microbial banks, and animal germplasm resource banks. Modern molecular detection methods can establish advanced plant genomics and seed biology experimental research platforms for germplasm resources.
Quantitative PCR (Quantitative Polymerase Chain Reaction) has a wide range of applications in germplasm resource research. The application of quantitative PCR in this field mainly includes the following aspects:
1. Genotype identification: Quantitative PCR can be used to quickly and accurately identify the genotype in germplasm resources. Detecting the presence or absence of the target gene and its expression level through PCR reaction can help researchers determine the useful genotypes contained in the germplasm resources and provide important information for subsequent genetic improvement work.
2. Gene expression analysis: Quantitative PCR can quantitatively detect the expression level of specific genes in germplasm resources. By comparing the expression of target genes in different germplasm resources or under different treatment conditions, the regulatory mechanism of genes in the process of growth and development can be revealed, providing a basis for selecting excellent genotypes.
3. Genetic improvement: Fluorescence real-time quantitative PCR technology can be used for molecular breeding. By analyzing the genome sequence and genetic characteristics of animals and plants, their growth, reproduction and other traits can be predicted, helping breeders to select excellent individuals for breeding and cultivation
4. Genetic diversity assessment: Quantitative PCR can be used to assess the genetic diversity of germplasm resources. By analyzing the genetic variation of multiple loci in germplasm resources, the genetic background and diversity level of germplasm resources can be determined, providing a scientific basis for the protection and rational use of germplasm resources.
5. Resistance identification: Quantitative PCR can be used to detect resistance to stress factors such as pests and diseases in germplasm resources. By detecting genes or markers related to resistance through PCR reactions, germplasm resources with resistance characteristics can be screened out to provide candidate materials for resistance breeding.
Aperbio has developed an international standard qPCR system for scientific research users - Pangu Rapid Quantitative PCR System, which follows the international MIQE principle, has outstanding performance and is very suitable for germplasm resource research.
• The heating speed can reach 8.5℃/s, and 96-well detection can be completed quickly and the results can be issued at one time, with high product yield and good specificity
• The temperature control accuracy of ±0.1℃ meets the stringent requirements of sensitive samples for amplification temperature and ensures the normal amplification of positive targets
• Supports 12-column temperature gradient function, which is convenient for rapid optimization of amplification conditions
• Optical waveguide top detection, no edge effect and optical path difference, no calibration required
• 6-color detection channels, support various commonly used fluorescent markers, compatible with various commercial qPCR kits
• It has powerful software functions for gene expression and genotyping, suitable for SNP/KASP and other allele genotyping
In general, the application of quantitative PCR in germplasm resource research can help researchers understand the genetic characteristics, gene expression, resistance and other important information of germplasm resources more comprehensively and deeply, and provide technical support for crop genetic improvement and rational use of germplasm resources.