naica® fully automated droplet digital PCR system

Naica® fully automated droplet digital PCR system

Naica® fully automated droplet digital PCR system uses top-notch microfluidic chips as consumables. It only requires one step of manual loading, and divides the sample into a single-layer droplet array in the chip through a restricted gradient. The droplets are visually read and quality controlled, and intelligent software is used for analysis. Naica® fully automatic droplet chip digital PCR system is the next-generation nucleic acid absolute quantification technology developed by Stilla, France.

Naica® fully automated droplet digital PCR system is the next generation of nucleic acid absolute quantification technology developed by Stilla, France. Stilla is a European biotechnology company headquartered in Paris. With a global team of interdisciplinary expertise, it uses advanced microfluidics chemistry, molecular biology and computer science technologies, and has more than 80 global patents. It is committed to providing breakthrough and flexible naica® systems to accelerate the development of next generation genetic testing. It provides high-precision genetic analysis solutions to researchers and clinicians around the world to improve health.

Workflow

· Only one manual loading operation is required to add the reaction solution to the Sapphire chip or Opal chip, and the rest of the steps are completed automatically;

· The chip is sealed throughout the process to prevent contamination;

· Automatic integrated droplet generation and PCR amplification;

· A single sample can reach ~30,000 droplets, with a wider dynamic range;

· Up to 48 samples can be processed simultaneously at one time;

· Simply transfer the chip to Prism3;

· Simultaneous three-fluorescence channel detection;

· The experimental data can be read in 10 minutes;

· User-friendly Crystal Miner software automatically analyzes data;

· Intuitive data result display and intelligent visual data analysis;

Sapphire and Opal chips are available to meet your different throughput detection needs. The results of both chips can be read repeatedly and the droplet stability is good.

Sapphire high sensitivity chip

▪ ~30,000 droplets/sample

▪ Volume 25ul

▪ 1-3 chips can be tested simultaneously, that is, 1-12 samples can be tested

▪ 4 sample wells per chip

Opal High-Throughput Array

▪ ~20,000 droplets/sample

▪ Volume 7ul

▪ 1-3 chips can be tested simultaneously, that is, 1-48 samples can be tested

▪ 16 samples per chip

Advantage

Easy to use, only one manual sample loading operation is required to obtain data

Automation Design, integrated droplet preparation and amplification saves 50% of space

Partition more, the number of effective droplets is twice as high as that of the second generation technology

Flexible throughput, 1 to 48 samples can be selected at a time

Advanced Multi, three fluorescence channels (blue/green/red) are detected simultaneously

Reading speed is twice as fast as second generation technology.Read quickly

Droplet visualization quality control and traceability analysis,Intelligent quality control

Compensation correction for visualizing fluorescence spillover,Fluorescence compensation

Enclosed in the chip to prevent contamination,Sealed design

PCR product recovery function in droplets,Droplet recovery

Crystal Miner Intelligent visual analysis software

▪ Automatically obtain graphical data results

▪ Intuitive visual data interface

▪ Wizard-style software design, easy to use

▪ Advanced functions to handle complex data analysis

Application and analysis