Kingwell Automated Thermal Cycler

Revolutionary PCR experience: with an ultra-fast heating rate (7℃/s), it saves your experiment time; better temperature accuracy and uniformity deliver excellent repeatability; and a powerful 750W instantaneous power addresses samples with high GC content and complex secondary structures. The Kingwell Automated Thermal Cycler is designed to simplify your experimental workflow and enhance result accuracy.

Overview

Ordering Information

Resources

Kingwell Automated Thermal Cycler, independently developed by Aperbio is a high-performance molecular biology device specifically designed to meet the needs for accurate and efficient nucleic acid amplification. This product series includes three models: Kingwell 96, Kingwell 96DW, and Kingwell 384, covering diverse experimental scenarios from basic to high-throughput applications.

Key Features

1. Precise Temperature Control System: It adopts a gold-plated block design with excellent thermal conductivity. The temperature control precision reaches ±0.1°C and the temperature uniformity is ±0.2°C, ensuring stable reaction conditions.

2. Efficient Heating: With a maximum heating rate of 7℃/s and an instantaneous power of 750W, it can quickly complete the amplification of samples with high GC content, complex secondary structures and low concentrations, reducing non-specific products.

3. Intelligent Operation: Equipped with a 10.1-inch color touchscreen, it supports presetting of multiple programs, simplifying the experimental process.

4. Automatic Hidden Chamber Loading/Unloading: It has good compatibility with consumables, reducing customers' consumable adaptation costs; it prevents evaporation, minimizing sample loss and experimental errors; and it supports automated integration, improving the efficiency of experimental operations.

5. It features a 12-column temperature gradient function with a span of 30°C, enabling quick identification of the optimal annealing temperature during experiments and enhancing experimental efficiency.

6. High-efficiency anti-condensation device, resisting operational risks in humid environments.

Application

● Pathogen Detection

● Gene Cloning

● NGS Library Preparation

● High-precision quantitative detection

● Amplification of Samples with High GC Content

● Optimization and Analysis Using Temperature Gradient

● Amplification of Crude Extract Samples

Specification

Model	Kingwell 96	Kingwell 96 DW
Temperature control module	Ultra-stable gold-plated module	Ultra-stable gold-plated module
Accuracy	±0.1°C	±0.1°C
Uniformity	±0.2°C	±0.2°C
Maximum ramp rate	7°C/S	5°C/S
Temperature gradient	12 columns, span 30°C	12 columns, span 30°C
Temperature range	4-100°C	4-100°C
Temperature range of the hot lid	30-110°C	30-110°C
Block format	96 well*0.2ml	96 well*0.2ml
Sample volume	5-80 μL, recommended 5-50 μL	5-150 μL，recommended 5-125 μL
Touch screen operation	10.1 inches	10.1 inches

Ordering Information

Catalog Number	Product name	Model	Description
611101	Automated Thermal Cycler	Kingwell96	0.2ml*96 well，maximum reaction volume 80ul
611102	Automated Thermal Cycler	Kingwell96DW	0.2ml*96 well，maximum reaction volume 150ul
611103	Automated Thermal Cycler	Kingwell384	384 well，maximum reaction volume 30ul

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