Digital PCR technology
Digital PCR
Digital PCR is an absolute quantitative nucleic acid molecule detection technology that achieves absolute quantification of target molecules by dispersing samples into thousands of independent micro-reaction units for PCR amplification, and then interpreting and analyzing the fluorescent signal of each unit. Compared with traditional real-time quantitative PCR, digital PCR can more accurately quantify the starting amount of target DNA, and is particularly suitable for the detection of rare mutants and the quantitative analysis of extracellular DNA.
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Higher sensitivity
Overcome the influence of homologous background nucleic acid and improve the detection rate
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Better precision
Identify even smaller concentration changes
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Highly tolerant to inhibitors
Applicable to a variety of samples to improve the positive detection rate
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Absolute quantification without calibration curve
Assignment/calibration of nucleic acid reference materials
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Higher data reproducibility
Reliability and comparability of data between batches