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naica® Droplet Chip Digital PCR System for Absolute Quantification of Dominant Bacteria Related to Water Quality Environment
In modern aquaculture, the water quality of the aquaculture system directly affects the health and production of fish. Microorganisms play a crucial role in the removal of organic matter and nitrogen cycling, as well as the production of toxic hydrogen sulfide (H2S). However, if microorganisms cause disease in fish or exhibit probiotic properties, it will directly affect the health of fish.
Recently, Stilla from France and SINTEF Ocean from Norway collaborated to publish an article titled "Absolute quantification of priority bacteria in aquaculture using digital PCR" in the Journal of Microbiological Methods, aiming to detect relevant advantageous bacteria in aquaculture.
In this paper, the author mainly analyzes three different bacteria related to salmon production:
The first type is fish pathogen, Moritella viscosa, which is associated with ulcerative diseases in fish, Yersinia rugkeri, which causes intestinal red mouth disease, and Flavobacterium psychohilum, which is associated with bacterial cold water disease in fish.
The second type is human pathogens that can be transferred from seafood to consumers, Listeria monocytogenes。
The third type is bacteria that threaten the health of fish by damaging the feeding environment. Sulfate reducing bacteria (SRB) typically affect fish health under anaerobic conditions by converting sulfate (SO42-) into toxic hydrogen sulfide (H2S). SRB detection can be performed using Desulfovibrio disulfuricans as a reference strain.
Researchers utilize naica ® The single and multiple detection methods of the droplet chip digital PCR system are used for absolute quantification of the above-mentioned advantageous bacterial strains. The results showed that the detection limits of Moritella viscosa, Yersinia ruckeri, and Flavobacterium psychohilum were around 20 fg, while the DNA detection levels of Listeria monocytogenes and Desulfovibrio disulfuricans could be as low as 2 fg, and they all had high linear dynamic ranges (Figure 1). The results of multiplex CDPCR detection were highly consistent with the concentration of the target gene detected in the corresponding single fold analysis (Figure 2, Figure 3). This experiment fully proves that naica ® The droplet chip digital PCR system can accurately quantify multiple dominant bacterial strains in complex water quality samples simultaneously.