Leave Your Message
AperTurbo Real-Time PCR Master Mix
Consumables and Assay

AperTurbo Real-Time PCR Master Mix

Take your qPCR experiments to the next level with AperTurbo qPCR Master Mix – engineered for ultra-fast cycling, high specificity, and reproducible results. Designed for researchers who demand both speed and accuracy, this master mix optimizes reaction efficiency while maintaining superior sensitivity across a wide dynamic range. 

    Overview

    Reduce run times without compromising data quality. AperTurbo accelerates workflows, helping you achieve results faster than ever.Designed to minimize non-specific amplification and primer-dimer formation, ensuring precise target detection even in complex samples.Every batch of AperTurbo is rigorously tested for lot-to-lot consistency, so you get reliable results every time.Compatible with most qPCR instruments and optimized for various probe- and dye-based assays.

    Key Features

    1

    Ultra-Fast Cycling for High-Quality Data

    Speed up your qPCR workflow without compromising data integrity. AperTurbo qPCR Master Mix is formulated for rapid thermal cycling, allowing researchers to obtain results in a fraction of the time compared to conventional master mixes. Despite its.
    accelerated protocol, AperTurbo ensures high specificity, sensitivity, and reproducibility, making it ideal for both demanding research applications and routine high-throughput testing.
    3

    Figure 1. Reproducibility and robustness challenge on the AperTurbo Green Master Mix. Amplicons size of 94 bp and 220 bp were tested on the ultra-fast thermal
    cycling protocol (35-min). The Master Mix were tested across 7-dynamic range from 5 × 10-107 per reaction.

    Proven Robustness for Reliable Performance

    AperTurbo qPCR Master Mix is engineered for stability and reliability, ensuring top-tier performance even under extreme conditions. To validate its robustness, we subjected AperTurbo to rigorous stress tests, including 7-day exposure at 50°C and 16 freeze-thaw cycles.
    In both scenarios, the master mix maintained consistent 99% PCR efficiency, demonstrating its resilience in demanding laboratory environments. Even after prolonged heat exposure, AperTurbo exhibited only a minimal, predictable 1 Ct delay, confirming its superior formulation and stability. These results not only reinforce the master mix’s durability but also provide confidence that it can be shipped at room temperature without compromising performance. Whether for routine testing or advanced research, AperTurbo ensures unwavering accuracy and reproducibility, every time.

    Housekeeping Gene

    Amplicon Size

    PCR Efficiency

    ACTB

    77

    96%

    B2M

    94

    95%

    RPLP0

    107

    102%

    GAPDH

    123

    99%

    ACTB

    77

    97%

    HPRT1

    98

    96%

    Figure 2. Six different human housekeeping genes were designed and tested on AperTurbo Green Master Mix across 7-dynamic range from 5 × 10-107 per reaction.

    Repeated Freeze Thaw

    3

    Figure 3. GAPDH Assay used performed across 7-dynamic range with Master Mixes exposed up to 7-day under 50ºC heat. All conditions demonstrated consistent 99% PCR efficiencies. There is an approximate shift of 1 CT value after 7-day. No signal were detected from NTC.

    Repeated Freeze Thaw

    4

    Figure 4. GAPDH Assay used performed across 7-dynamic range with Master Mixes exposed up to 16-freeze-thaw treatment. All conditions demonstrated consistent 99% PCR efficiencies.

    Ordering Information

    Real-Time PCR Instrument

    ROX Requirement

    Catalogue Number

    2X Green MM

    2X Probe MM

    Roche Lightcycler®

    Qiagen Rotor-Gene™

    Eppendorf Mastercycler® MIC qPCR

    Bio-Rad® iQ™5, CFX96, CFX384, Opticon

    Aperbio Pangaea

     

     

    Not required.

     

     

     

    150009006

    150009012

    150009024

     

    150010006

    150010012

    150010024

    Applied Biosystems® 7500, QuantStudio™ 3, 5 and 7

    Agilent Mx™

    ViiA7™

    Low ROX

    150011006

    150011012

    150011024

    Applied Biosystems® 7000, 7300

    7700, 7900HT, StepOne™, StepOnePlus™

     

    High ROX

    150012006

    150012012

    150012024

    150013006

    150013012

    150013024

    If you need any additional materials such as colour brochures, technical guides, professional literature and operation videos, please fill in the following information and contact us.