AperTurbo Real-Time PCR Master Mix
Overview
Key Features

Ultra-Fast Cycling for High-Quality Data
Proven Robustness for Reliable Performance
AperTurbo qPCR Master Mix is engineered for stability and reliability, ensuring top-tier performance even under extreme conditions. To validate its robustness, we subjected AperTurbo to rigorous stress tests, including 7-day exposure at 50°C and 16 freeze-thaw cycles.
In both scenarios, the master mix maintained consistent 99% PCR efficiency, demonstrating its resilience in demanding
laboratory environments. Even after prolonged heat exposure, AperTurbo exhibited only a minimal, predictable 1 Ct delay,
confirming its superior formulation and stability. These results not only reinforce the master mix’s durability but also provide confidence that it can be shipped at room temperature without compromising performance. Whether for routine testing or
advanced research, AperTurbo ensures unwavering accuracy and reproducibility, every time.
|
Housekeeping Gene |
Amplicon Size |
PCR Efficiency |
|
ACTB |
77 |
96% |
|
B2M |
94 |
95% |
|
RPLP0 |
107 |
102% |
|
GAPDH |
123 |
99% |
|
ACTB |
77 |
97% |
|
HPRT1 |
98 |
96% |
Figure 2. Six different human housekeeping genes were designed and tested on AperTurbo Green Master Mix across 7-dynamic range from 5 × 10-107 per reaction.
Repeated Freeze Thaw

Figure 3. GAPDH Assay used performed across 7-dynamic range with Master Mixes exposed up to 7-day under 50ºC heat. All conditions demonstrated consistent 99% PCR efficiencies. There is an approximate shift of 1 CT value after 7-day. No signal were detected from NTC.
Repeated Freeze Thaw

Figure 4. GAPDH Assay used performed across 7-dynamic range with Master Mixes exposed up to 16-freeze-thaw treatment. All conditions demonstrated consistent 99% PCR efficiencies.
Ordering Information
|
Real-Time PCR Instrument |
ROX Requirement |
Catalogue Number |
|
|
2X Green MM |
2X Probe MM |
||
|
Roche Lightcycler® Qiagen Rotor-Gene™ Eppendorf Mastercycler® MIC qPCR Bio-Rad® iQ™5, CFX96, CFX384, Opticon Aperbio Pangaea |
Not required. |
150009006 150009012 150009024 |
150010006 150010012 150010024 |
|
Applied Biosystems® 7500, QuantStudio™ 3, 5 and 7 Agilent Mx™ ViiA7™ |
Low ROX |
150011006 150011012 150011024 |
|
|
Applied Biosystems® 7000, 7300 7700, 7900HT, StepOne™, StepOnePlus™ |
High ROX |
150012006 150012012 150012024 |
150013006 150013012 150013024 |
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